Cyanocobalamin (Vitamin B12)

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Cyanocobalamin (Vitamin B12 USP) 


  • CAS Number: 68-19-9
  • PubChem CID: 16212801
  • DrugBank: DB00115
  • ChemSpider: 24921423
  • UNII: P6YC3EG204
  • ChEMBL: ChEMBL2110563
  • ECHA InfoCard: 100.000.618

Physical and Chemical Properties:

Assay (on dried basis): 96.00% ~ 100.50%

Molecular formula: C63H88CoN14O14P
Molecular weight: 1,355.38 g/mol
Melting point: 300 °C (572 °F) +
Solubility: Soluble in water (50 mg/ml), DMSO (75 mg/ml at 25 °C), and ethanol (10 mg/ml at 25 °C). Insoluble in chloroform, and acetone.

Storage and Transport:

Stored in cool and airy place; away from fire and heat; handle with care; no breakage, avoid leakage. Please refer to SDS for more information about Safety, Storage and Transportation.


Dhc cultures require the cobalt-containing transition-metal coenzyme vitamin B12.  It is reported that optimal dechlorination and growth occur at vitamin B12 concentrations ranging from 25 to 50 micrograms per liter (25 µg/L) (Stroo et al., 2013).   Vitamin B12 is not commonly found in simple substrates such as emulsified vegetable oil (EVO) and at considerably lesser amounts in micronutrient blends.  To answer the growing demand for vitamin B12 and to provide for flexibility in adding vitamins in the field, Tersus offers Cyanocobalamin (Vitamin B12 USP) packaged in 100 gram bags and 1 kg tins.


Stroo et al., 2013, Bioaugmentation for Groundwater Remediation, edited by Stroo, H.F., Leeson, A., Ward, C.H. HydroGeoLogic, Inc., Ashland, OR, USA